Detection of a Small Number of Cryptosporidium parvum Oocysts by Sugar Floatation and Sugar Centrifugation Methods
نویسندگان
چکیده
منابع مشابه
Recovery and detection of Cryptosporidium parvum oocysts from water samples using continuous flow centrifugation.
Continuous flow centrifugation (CFC) was used in conjunction with immunomagnetic separation (IMS) and immunofluorescence microscopy (IFA) and nested PCR to recover and detect oocysts of Cryptosporidium parvum and cysts of Giardia intestinalis from 10L volumes of source water samples. Using a spiking dose of 100 oocysts, nine of 10 runs were positive by IFA, with a mean recovery of 4.4+/-2.27 oo...
متن کاملInactivation of Cryptosporidium parvum Oocysts by Ammonia.
The survival of Cryptosporidium parvum oocysts in soil and water microhabitats may be affected by the environmental production and release of free ammonia. The objective of this study was to determine the effects of increasing free ammonia concentrations and times of exposure on oocyst viability. Wild-type oocysts were obtained from naturally infected calf feces by chemical (continuous-flow) ce...
متن کاملRotifers ingest oocysts of Cryptosporidium parvum.
Six genera of rotifers including Philodina, Monostyla, Epiphanes, Euchlanis, Brachionus, and Asplanchna were exposed to oocysts of Cryptosporidium parvum cleaned of fecal debris. Unstained oocysts and those stained with fluorescein-conjugated monoclonal antibody were added to suspensions of viable rotifers and were examined by phase-contrast, differential interference contrast, and fluorescence...
متن کاملisolation of small number of cryptosporidium parvum oocyst using immunochromatography.
background: cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. the organism can be transmitted through water. since small number of c. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of c. parvum oocyst in samples with limited number of oocysts. methods: antibody was prepared against ...
متن کاملDetection of Cryptosporidium parvum oocysts on fresh vegetables and 1 herbs using antibodies specific for a Cryptosporidium parvum viral antigen.
The purpose of this study was to determine if the viral symbiont of Cryptosporidium parvum (CPV) sporozoites could be used as a target for sensitive detection of the parasite in food samples. Polyclonal sera specific to the recombinant viral capsid protein (rCPV40) was used in a dot blot hybridization assay to detect oocysts recovered from green onions and cilantro. Small batches of chopped gre...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2006
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.68.1191